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. 2022 Apr 27;13(3):e00395-22. doi: 10.1128/mbio.00395-22

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Copyright © 2022 Reslane et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Complementation with arcB1 or arcA1B1D1C1 rescues the growth of JE2 in CDM-R. (A and B) Growth analysis of JE2/pNF378, JE2/pNF379, and JE2/pNF407 complementation plasmids harboring the arcA1B1D1C1 operon, the argGH operon, and arcB1, respectively, in CDM-R. Growth of JE2/pBK123 (empty vector) in CDM and CDM-R was used as a control. (C to F) Growth of JE2 and JE2 putA in increasing concentrations of ornithine (C and D) and citrulline (E and F), respectively. (G) qRT-PCR assessing the transcripts of argF and arcB1 in WT JE2 in both CDM with ornithine and CDM-R with ornithine. Data are representative of results from three independent biological replicates performed with two technical replicates in each experiment. Error bars show means ± SEM (n = 3). Statistical significance was assessed using one-way ANOVA followed by Dunnett’s posttest; *, P < 0.05. (H) Growth of JE2, JE2 ccpA, JE2 proC, and JE2 rocA in CDM-R. Growth data in panels A to F and H represent results from three technical replicates per strain. Data are represented as means ± SEM.