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. 2022 May 21;7(1):ysac008. doi: 10.1093/synbio/ysac008

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of trypsinization of Mcap cells on the production of mRNA and eGFP protein in CFE reactions. (A) E. coli CFE of eGFP, mixed with trypsin-Mcap lysate or S30B buffer at different ratios. We observed a decrease in eGFP expression >75 vol% of either S30B buffer or trypsin-Mcap lysate. (B) Transcription of Spinach2 aptamer in trypsin-Mcap lysate. The fluorescence signal from the Spinach2-DFHBI-1T complex was detected, which indicated mRNA production. However, translation was not observed in CFE reaction containing 100% trypsin-Mcap lysate.