Figure 7.

Comparative RNase activity among several mycoplasma lysates confirmed a higher RNase activity in Mcap lysate compared to Syn3A. The mRNA substrate (1.5 µg) was incubated at 30°C with mycoplasma lysate (protein concentration ∼3 mg/ml) for 1–10 min. RNA samples were analyzed using denaturing 1% agarose gel electrophoresis. The high rRNA content in the digitonin-Syn3A lysate suggested the presence of intact ribosomes. Mcap, Syn3A and trypsin-Syn3A lysates were prepared by sonication and digitonin-Syn3A lysate by digitonin treatment.