Table 1.

Conditions tested for the cell lysis step for preparing mycoplasma lysates

Lysis method	Lysis condition	No. of cycles	Run-off incubation	Dialysis
Sonication	20%, 35%, 40%, 50% amplitude	1, 2, 4, 5, 7 (30 s on, 30/60 s off)	±	±
Osmotic shock	250 mM NaCl	2 washes, resuspend (30 min, 23°C)	+	+
Liquid nitrogen grinding	Grinding flash-frozen pellet	Liquid nitrogen added every minute (for 20 min)	+	–
French press	1000, 2000 psi	1, 3	±	±
Surfactant-based	1 vol% TX100 1 mg/ml digitonin	1–3 cycles (15 min, ice)	–	+