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(A) Schematic of the generation of the SCi-NCCIT line (created with BioRender.com). (B) Cas9 immunoblot displaying activation of dCas9-KRAB 72 hours post-doxycycline induction. (C) H3K9me3 ChIP-seq heatmap of the SCi-NCCITs shows increased H3K9me3 signal post-doxycycline treatment. (D) Genome browser screenshot displaying increased H3K9me3 before and after treatment with doxycycline at a de-repressed SVA-D.
