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. 2022 Jun 15;18(6):e1010225. doi: 10.1371/journal.pgen.1010225

Fig 4. CRISPRi-mediated repression of conventionally de-repressed SVAs results in aberrant gene expression.

Fig 4

(A) Volcano plot showing genes differentially expressed in SCi-NCCITs after doxycycline treatment (purple = downregulated genes; green = upregulated genes). (B) Heatmap of 131 genes that are differentially expressed post-doxycycline treatment and also represent the nearest gene to an SVA. (C) Top canonical pathways predicted by IPA for the 131 genes differentially expressed after doxycycline treatment. (D) Canonical pathways predicted by IPA for the 111 downregulated (red) and 20 upregulated (green) genes. (E) Top upstream regulators/transcription factors predicted by IPA for the 131 genes differentially expressed after doxycycline treatment.