Figure 7.

Inhibition of Retn blocks myocardial apoptosis. TUNEL staining was performed on LV tissues from the different groups at 10 weeks post-TAC as indicated. (A) TUNEL-positive apoptotic cells are shown as red-stained cells under fluorescent microscope. (B) Quantification of apoptosis shown as a percentage of apoptotic nuclei (red by TUNEL) vs. total nuclei (blue by DAPI) (scale bar, 100 µm, n = 4, 5–6 random fields/group were quantified). Values are shown as mean ± S.E.M. Significance was evaluated by Student’s t-test or one-way ANOVA with Tukey’s post-hoc test. P values <0.05 were considered significant.