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. 2022 Jun 28;11:e78203. doi: 10.7554/eLife.78203

Figure 5. Ddx3x-depleted progenitors exhibit prolonged cell division and more proliferative divisions at the expense of neurogenic divisions.

Figure 5.

(A) Live imaging paradigm for monitoring cell fate. Figure 5A has been adapted from Figure 3A and E from Pilaz et al., 2016. (B) Live imaging DIC snapshots at indicated t=minutes or hours, and fixed images stained with indicated markers. (C) Quantification of re-divisions (black) and 1 division (white) in control and cHet F/cKO M. n=304 (control) and 539 (cHet F/cKO M) total cells. (D) Schematic illustrating an example of a re-division. (E) Quantification of cell fate for P,P divisions (2 Sox2+ RGCs, or 2 Tbr2+ IPs, or 1 Sox2+ RGC and 1 Tbr2+Tuj1- IP); P, N divisions (1 Tuj1+ neuron and either 1 Sox2+ RGC or 1 Tbr2+ IP); N, N divisions (2 Tuj1+ neurons). n=>70 cells/condition/trial with three trials. Scale bars, indicated. Error bars, S.D. *p<0.05, ****p<0.0001. Two-tailed Fisher’s exact test (C), Two-way ANOVA with Sidak’s correction (E).