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. 2022 Jun 28;11:e78203. doi: 10.7554/eLife.78203

Figure 7. DDX3X-dependent translation target, Setd3, is required for neurogenesis.

Figure 7.

(A) RT-qPCR quantification of Setd3 knockdown in N2A cells. n=4/condition with two independent trials. (B) Representative sections of E17.5 brains from mice electroporated at E14.5 with pCAG-GFP and scrambled or Setd3 siRNAs and immunostained with GFP (grey), Sox2 (magenta), Neurod2 (green). (C) Quantification of distribution of GFP-positive cells in 5 even bins of cortex. n=4 embryos/condition. (D, E) Quantification of GFP co-localization with Sox2+ (D) and Neurod2+ (E) cells. n=4 embryos/condition. (F) Schematic model summarizing how loss of DDX3X-dependent translation impairs neurogenesis. Scale bars, indicated. Error bars, S.D. *p<0.05, ****p<0.0001. Student’s unpaired, two-tailed t-test (A, D, E), Two-way ANOVA with Sidak’s correction (C).