Table 1. Definition of optimized parameters.

Parameter names, parameter name codes, description, parameter ranges, parameter units, correspondence between experimental procedure and parameters used (related to Figures 2A, 3A and B).

Parameter name	Code	Description	Range	Unit	Protocol step
Preconditioning concentration	PC	FGFRi concentration in medium	0–505	nM	Preconditioning
Preconditioning period	PP	FGFRi duration in medium	1–6	day	Preconditioning
Detachment trypsin period	DP	Trypsin incubation duration at room temperature after incubation at 37 °C, 20 min.	5, 8, 11, 14, 17, 20, 23	min	Passage
Detachment pipetting strength	DS	Pipetting strength during cell detachment	10–100	mm/s	Passage
Detachment pipetting length	DL	Bottom surface area to be pipetted	short / long	N/A	Passage
KSR period	KP	KSR concentration and duration in medium: KSR concentration is decreased linearly every day so that KSR becomes 10% on DDday of KP value	1–19	day	RPE differentiation
Three supplements period	3P	Three chemical supplements duration	3–19	day	RPE differentiation