Table 1. List of protein expression constructs used in this study.
| ID | Construct | Amino acids | Vector | Tag (N-terminal) | Tag (C-terminal) |
|---|---|---|---|---|---|
| RN82 | full length | 1–1472 | pFastBac1 | none | 6× His |
| RN63 | CC1-CKK | 696–1,454 | pGFPS1 | HAT*-GFPS1-TEV* | 7× His |
| RN64 | CC3-CKK | 1157–1454 | pGFPS1 | HAT*-GFPS1-TEV* | 7× His |
| RN136 | CKK | 1332–1472 | pGFPS1 | HAT*-GFPS1-TEV* | 7× His |
| RN189 | full length | 1–1472 | pFastBac1 | His_Strep2-3Csite | none |
| RN282 | CC1-CKK-CT | 696–1472 | pET28 | His_Strep2-3Csite | none |
| RN290 | CC3-CKK-CT | 1157–1472 | pET28 | His_Strep2-3Csite | none |
| RN318 | ∆CKK | 1–1331 | pFastBac1 | His_Strep2-3Csite | none |
| RN328 | ∆CC3 | 1–1472 ∆1154–1250 | pFastBac1 | His_Strep2-3Csite | none |
*
These tags carry modifications. See the Methods for details.