Fig. 3. Global proteomics profiling of extracellular vesicles.

a Schematic diagram of SILAC labelling and extracellular vesicles (EV) isolation. b log₂[H/L] dotplot of EV proteins that had SILAC ratios in forward (WT heavy; ATG7-KO light) and reverse (ATG7-KO heavy; WT light) labelling experiments. Grey lines denote a 1.5× fold enrichment cutoff. c Heatmap of proteins identified to have a >1.5 fold enrichment when comparing ATG7-KO to WT EVs in both forward and reverse labelling experiments. d Gene ontology analysis (cellular compartment) of proteins that were enriched >1.5 fold in EVs of ATG7-KO vs. WT, plotted according to −log₁₀FDR.