Skip to main content
. 2022 Jun 28;13:3720. doi: 10.1038/s41467-022-31213-7

Fig. 5. ATG7-independent mitochondria secretion occurs via secretory autophagy.

Fig. 5

a Proteinase K protection assay of homogenates from WT or ATG7-KO cells stably expressing mCherry-Parkin treated with A/O and BafA1 (200 nM) for 12 h in the presence or absence of SAR405 (5 µM). b Quantification of the percentage of NDP52 protected from proteinase K digestion for each treatment condition. Mean of n = 3 independent replicates ±SEM is shown. P values were calculated by two-way ANOVA followed by Dunnett’s multiple comparisons test against the A/O + BafA1 group. *P  <  0.05, **P  <  0.01, ***P  <  0.001, and ns denotes not significant. See source data for exact P values. c Representative TEM images of mature autophagosomes containing mitochondria from WT or ATG7-KO cells stably expressing mCherry-Parkin treated with A/O + BafA1 for 16 h. Scale bar represents 500 nm. d ATG7-KO and ATG14/ATG7 DKO; e ATG7-KO and FIP200/ATG7 DKO; f ATG7-KO and ATG9A/ATG7 DKO; g ATG7-KO and SNAP23/ATG7 DKO; h or ATG7-KO and RAB7A/ATG7 DKO HeLa stably-expressing mCherry-Parkin were treated with A/O for 24 h. Extracellular vesicles (EVs) were isolated by differential ultracentrifugation and immunoblotted for the indicated proteins.