Table 2.
PMCA results and limit of detection in tissues from selected sheep with positive PMCA in PSLN.
| Group | Sheep ID | Component transfused | Survival period (days post infection) | Tissues tested No. replicates positive/No. tested (Limit of detection*) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Prescapular LN | Mesenteric LN | Peyer’s Patch | Tonsil | Spleen | Brain | Buffy coat | ||||
| 1 | P241 | Red cells | 333 | 3/3 (10–4) | 0/3 (NA) | NT | 3/3 (NT) | 3/3 (10–3) | 3/3 (10–3) | 0/3 (NA) |
| 2 | P225 | Buffy coat | 503 | 3/3 (10–1) | 0/2 (NA) | 2/2 (10–2) | 2/2 (10–1) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) |
| P243 | Buffy coat | 2323 | 3/3 (10–2) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | |
| P248 | Whole blood | 2102 | 3/3 (10–1) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | |
| P353 | Buffy coat | 2109 | 3/3 (10–1) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | 0/2 (NA) | |
| 3 | P467 | LR-RCC | 573 | 3/3 (10–1) | 2/3 (10–1) | NT | NT | 3/3 (10–2) | 3/3 (10–2) | 0/3 (NA) |
| P304 | LR-PCAPT-RCC | 2220 | 0/3 (NT)† | NT | NT | 3/3 (10–2) | 3/3 (10–2) | 3/3 (10–3) | 0/3 (NA) | |
| Q397 | LR-PCAPT-RCC | 3476 | 3/3 (10–1) | 0/3 (NA) | 0/3 (NA) | 0/3 (NA) | 0/3 (NA) | 0/3 (NA) | 0/3 (NA) | |
NA not applicable, NT not tested.
*Highest dilution of tissue homogenate giving positive PMCA result.
†Positive in several other experiments for 10–1 dilution (see Table S2).