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. 2022 Jun 28;13:3701. doi: 10.1038/s41467-022-31282-8

Fig. 5. Lsm7 undergoes phase separation in vitro.

Fig. 5

a Lsm7-GFP was analyzed for the formation of condensates at room temperature with or without the addition of 20% Dextran 70 (pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm. b Lsm7-GFP phase-separated condensates are concentration-dependent (pH 7.4, NaCl 150 mM, 20% Dextran 70). Images show representative data from three independent experiments. Scale bar, 2 μm. c Lsm7-GFP condensates (10 µM) are pH and salt sensitive. 20% Dextran 70 was added to all except for the first row. Images show representative data from three independent experiments. Scale bar, 2 μm. d Lsm7-GFP, lsm7ΔIDR-GFP and lsm7Δ90-103-GFP (5 µM) were analyzed for the formation of condensates with or without the addition of 10% Dextran 70 (pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm.