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. 2022 Jun 28;13:3701. doi: 10.1038/s41467-022-31282-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Prior to stress treatment, Lsm7-GFP and Pab1-RFP are diffusely distributed in the cytosol. b Upon 2-DG treatment, Lsm7 quickly phase separates, creating condensates in conjunction with available RNA and supported by PB components. c Pab1 is recruited and starts to demix, aided by PBs and/or specific PB components. The subsequent SGs associate on the side of the Lsm7 condensates. d Over time SGs increase in size whereas the Lsm7 condensates remain similar in size. e Lsm7 condensates might not be needed for maintenance of already formed SGs, and SG core maturation. Hence, it might be that Lsm7 condensates and SGs dissociate (indicated by dotted arrows). Created with BioRender.com.