Fig. 2.

ALKBH5 facilitates neutrophil accumulation in the site of infection in antibacterial defense. a FACS analysis of neutrophils in the peritoneal cavity of WT mice operated sham or mild CLP for 36 h. b Absolute numbers of peritoneal neutrophils from mice as in (a) (n = 8). c FACS analysis of neutrophils in the peritoneal cavity of Alkbh5-deficient mice and WT littermates at 36 h after mild CLP (n = 10). d FACS analysis of macrophages in the peritoneal cavity of mice as in (c) (n = 10). e FACS analysis of apoptotic neutrophils in the peritoneal cavity of mice as in (c) (n = 10). f FACS analysis of apoptotic neutrophils in the bone marrow of Alkbh5-deficient mice and WT littermates in the steady state (n = 3). All data are mean ± SEM of biologically independent samples. Data are representative of 8–10 independent experiments with similar results [(a) and (c)]. *P < 0.05; ****P < 0.0001; ns, not significant. Two-tailed unpaired Student’s t test (b–f)