Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 28;8:61. doi: 10.1038/s41421-022-00407-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a UMAP of YFP⁺ cells (excluding neurons and pericytes) labeled by pseudotime value with trajectory from Monocle 3. b UMAP of YFP⁺ cells (excluding neurons and pericyte) labeled by embryo harvest time. c UMAP of YFP⁺ cells (excluding neurons and pericyte) labeled by identity. d Z-score heatmap of gene expression in different branches, where rows are genes and columns are cells ranked by pseudotime value. Genes were first fit with generalized additive model (GAM) with ranked pseudotime as independent variable. Genes with the most significant time-dependent model fit (lowest P-value) were extracted and clustered by hierarchical clustering. Cells were ordered according to scaled pseudotime value from 0 to 1. Left, Myogenic lineage from EP to EM and LM; Middle, Dermal lineage from LP to BA; Right, Brown adipogenic lineage from LP to DF. e Scatter plot of temporally expressed genes obtained from GAM to scaled pseudotime. LOESS line of expression level was shown for each gene.