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. 2022 Jun 28;8:61. doi: 10.1038/s41421-022-00407-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Violin plot of Wnt5a expression in Smart-seq2 and 10× datasets. b Scheme of Wnt5a treatment on embryonic progenitor cells. E14.5 embryos were harvested, and both Pdgfra⁺/Thy1⁻ and Pdgfra⁺/Thy1⁺ populations were sorted. Cells were treated with 2.5 ng/mL bFGF to reach confluency. Wnt5a was then applied and 48 h after treatment, cells were harvested for analysis. c Embryonic cells were harvested and subjected to Western blot assay. Ponceau S staining was used as loading controls. d RT-qPCR analysis of Wnt5a-treated embryonic progenitor cells in b. One-way ANOVA was performed on Pdgfra⁺/Thy1⁻ cultured cells, followed by Dunnett’s multiple comparisons test. Student’s t-test was performed in Pdgfra⁺/Thy1⁺ cultured cells. Values are means ± SEM, n = 3; ns, not significant; **P < 0.01, ***P < 0.001.