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. 2022 Jun 28;13(6):573. doi: 10.1038/s41419-022-05005-2

Fig. 3. Ionising radiation (IR) induced mitotic catastrophe after downregulation of glycogen phosphorylase liver isoform (PYGL).

Fig. 3

A, B Immunofluorescence analysis (A) and quantitative analysis of γH2AX foci (B) following IR in control (shControl) and PYGL knockdown (shPYGL) U87MG cells. C Western blot analysis of γH2AX, p21, pRPA in response to IR in shControl and shPYGL U87MG cells. D Inhibition of PYGL induces mitotic catastrophe, DNA fragmentation and a giant cell phenotype following IR (blue arrows). Scale bars 20 μm. E Representative still images of a time relapse video 72-96 h after IR showing mitotic catastrophe during cell division in PYGL knockdown cells after IR. Scale bars 20 μm. F Representative senescence staining following IR in shControl and PYGLkd U87MG cells. SPiDER-βGal (1 μM) and fluorescence live microscopy was performed (excitation 450–490 nm, emission 500–550 nm) using the confocal spinning disc. Scale bars 20 μm.