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. 2022 Jun 28;13(6):573. doi: 10.1038/s41419-022-05005-2

Fig. 5. Inhibition of glycogen phosphorylase liver isoform (PYGL) alters the metabolic profile of glioblastoma (GBM) cell lines in response to ionising radiation (IR).

Fig. 5

A Western blot analysis of phosphorylated AMP-activated protein kinase (pAMPK) and its downstream target phosphorylated acetyl-CoA carboxylase (pACC) in control (shControl) and PYGL knockdown (shPYGL) U87MG cells 24 h, 48 h and 5 days after 12 Gy IR. B, C Basal and maximum oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) are lower in shPYGL than shControl cells 48 h and 5 days after IR. (n = 3, error bars are ±SD, ***p < 0.001, p values were calculated by Two Way ANOVA - Tuckey post-hoc).