TABLE 1.
Strains of E. coli used in the inoculum cocktail
| Strain | Pathotype | Serogroup | Virulence determinant(s)a | Source | Reference(s) | Selective mediumb/ carbohydrate reaction |
|---|---|---|---|---|---|---|
| 86-24 | STEC | O157:H7 | eae, Stx2 | Human | 15, 23 | SN dulcitol/positive |
| 3081 | STEC | O157:H7 | eae, Stx1, Stx2 | Bovine | 11, 13 | KA sorbitol/negative |
| 2041 | ETEC | O157:H43 | F4, F41, LT, STb | Porcine | 52 | TN sorbose/positive |
| 637 | ETEC | O64:NM | F5, STa | Porcine | 43, 54 | TN sorbose/negative |
| E2348/69 | EPEC | O127:H6 | eae | Human | 39, 44 | SN dulcitol/negative |
a
LT, heat labile enterotoxin; STb, heat-stable enterotoxin b; STa, heat-stable enterotoxin a; eae, gene encoding intimin; F4, F4 (K88) fimbriae; F5, F5 (K99) fimbriae; F41, F41 fimbriae.
b
MacConkey agar base; S, streptomycin (100 μg/ml); N, nalidixic acid (10 or 20 μg/ml); T, tetracycline (10 μg/ml); K, kanamycin (30 μg/ml); A, ampicillin (100 μg/ml).