Figure 2.

TA inhibits CG-induced EMT and ECM protein deposition in the fibrotic peritoneum. (A) Western blot analysis showed the protein levels of α-SMA, Collagen I, Fibronectin, E-cadherin, and GAPDH in peritoneum from different groups of mice. Expression levels of (B) α-SMA, (C) Collagen I, (D) Fibronectin, and (E) E-cadherin in different groups were quantified by densitometry and normalized with GAPDH. (F) Representative micrographs of immunofluorescence staining of α-SMA and quantization count of α-SMA-positive cells. (G) Representative micrographs of immunohistochemical staining of Collagen I and quantization of Collagen I-positive area (%). (H) Western blot analysis showed the protein levels of MMP2, MMP9, and GAPDH in peritoneum from different groups of mice. Expression levels of (I) MMP2 and (J) MMP9 in different groups were quantified by densitometry and normalized with GAPDH. Data were expressed as means ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. All scale bars = 20 μm. NS: P≥0.05.