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. 2022 Jun 15;4:909943. doi: 10.3389/fmedt.2022.909943

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Copyright © 2022 Rossi and Blasi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of NP size and shape on MCTS uptake and penetration. (A,B) Effect of NP size: uptake and penetration of spherical 100, 200, and 500 nm polystyrene particles in Mitomycin C-treated HEK 293 spheroids. (A) Graph showing comparison of normalized pixel intensity associated with spheroid per slice with different diameter polystyrene beads and (B) Normalized radial intensity distribution of polystyrene beads as a function of distance from the center of the spheroid. (C) Effect of NP shape: normalized radial intensity distribution of shape-specific nanoparticles as a function of distance from the center of the spheroid. Only positive error bars are shown, to allow proper visualization of data. Reproduced with permission from Agarwal et al. (43) (John Wiley and Sons License, license number 5306710208408).