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. 2022 Jun 15;13:907209. doi: 10.3389/fphar.2022.907209

FIGURE 4.

FIGURE 4

Urolithin A (UroA) promotes renal PINK1/Parkin-mediated mitophagy in fructose-fed mice. (A,B) Representative immunoblots of PINK1, Parkin, p62, and LC3 I/II protein levels in mouse kidneys (A) and densitometric analysis (B). β-actin was used as an internal reference. (C) Representative images of immunofluorescence staining of LC3, LAMP1, and TOMM20 of kidney tissue sections (Red, LC3; Purple, LAMP1; Green, TOMM20). Original magnification: ×400; Scale bar: 20 μm. (D–F) Densitometric analysis of LC3 (D), LAMP1 (E), and TOMM20 (F). (G) Representative transmission electron microscopy (TEM) images in the kidney tissues from the wild-type (WT) or fructose-fed mice with intragastric administration of either vehicle or UroA, respectively. N: nucleus; M: mitochondria; autolysosomes (red arrow). Mean ± S.D., n = 5. # p < 0.05, ### p < 0.001 versus the WT group; * p < 0.05, ** p < 0.01, *** p < 0.001 versus the fructose group. UroA-L and UroA-H represent intragastric administration of urolithin A at low (50 mg/kg/day) and high (100 mg/kg/day) doses, respectively.