FIG. 1.

Determination of N glycosylation of CY047 and CY181. Ten-microliter aliquots of supernatant of YPGal-grown yeast cultures, containing the respective cutinase variants, were subjected to cleavage of the attached sugars by using the endo H deglycosylation kit (catalog no. 1 836 579; Boehringer Mannheim) according to the manufacturer's instructions. The proteins were separated on an SDS–12% PAGE gel. As a control CY000, CY047, and CY181 cutinases without endo H treatment were loaded on the gel.