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Pulse-chase analysis of cutinase (glyco)variants. Cutinase-expressing cells were labeled for 10 min with 15 μCi of [35S]methionine-cysteine for 10 min. At the indicated time points samples were taken and prepared for immunoprecipitation as described in Materials and Methods. Precipitated complexes were subjected to SDS-PAGE, and autoradiography was performed.
