FIG. 4.

Secretion levels of (glycosylated) antibody fragments by S. cerevisiae. Yeast cells transformed with the indicated antibody fragment construct were grown overnight in yeast extract–peptone–d-glucose diluted 1:10 in YPGal for induction of the antibody fragment gene. After 24 h, antibody fragment production was measured by subjecting 10 μl of culture supernatant to SDS-PAGE with or without treatment with the endo H deglycosylation kit (catalog no. 1 836 579; Boehringer Mannheim) according to the manufacturer's instructions. Symbols: ★, glycosylated antibody fragment; ●, unglycosylated antibody fragment.