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. 2022 Jun 15;13:895519. doi: 10.3389/fimmu.2022.895519

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Copyright © 2022 Zauhar, Biber, Jabri, Kim, Hu, Kaplan, Pfaller, Schäfer, Enzmann, Schlötzer-Schrehardt, Straub, Hauck, Gamlin, McFerrin, Messinger, Strang, Curcio, Dana, Pauly, Grosche, Li and Stambolian

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complement proteome of macroglia (Müller cell, astrocytes), retinal neurons and RPE/choroid in the macular and peripheral human eye. Müller cells and retinal neurons were purified from peripheral and macular retinal tissue punches (6 mm in diameter) from 5 donor retinae ( Table S1 ) by magnetic-activated cell sorting (MACS) and were submitted to quantitative LC MS/MS mass spectrometry. Contamination with astrocytes is likely, because no surface marker yet separates them clearly from Müller cells. Müller cells definitely outnumber astrocytes. Macroglia and neurons were depleted from ITGAM (alias CD11B)-positive microglia/macrophages and CD31-positive vascular cells. RPE/choroid was collected after the removal of retinal tissue and comprised a mixture of RPE and choroidal cell types including pericytes, endothelial cells, fibroblast and immune cells. Given the intense perfusion of choroidal tissues, these samples do not allow unequivocal discrimination of the source especially of soluble complement components as they could be expressed by local cell types or by liver cells and enter the choroid via the circulation.