Figure 1.

Long-term donor chimerism is established by mobilization-based HSCT (M-HSCT)

(A) Schematic of the M-HSCT protocol. Recipient CD45.2 mice were mobilized with G-CSF (green dots) and AMD3100 (red triangle), with and without BIO5192 (blue triangle) (G7A; G7AB), and subsequently transplanted with CD45.1 2 × 10⁶ Lin⁻ cells, collected from the BM.

(B) Counts of mobilized WBC (left panel), LSK (middle panel), and SLAM HSC (right panel) per mL in the PB in non-mobilized (Sham) and G7A and G7AB mobilized mice.

(C) Counts of LSK cells per million of Lin⁻ CD45.1 donor cells, collected from the BM.

(D) Long-term follow-up of the donor CD45.1 (blue) and recipient CD45.2 (ocher) chimerism observed within total CD45⁺ cells in PB after transplanting 2 × 10⁶ Lin⁻ cells post-mobilization in recipient CD45.2 mice.

(E) The reconstitution of myeloid and lymphoid lineages over time of the recipient CD45.2⁺ cells (left panel) and CD45.1⁺ cells (right panel) in the PB of recipient CD45.2 mice.

(F–I) Chimerism of CD45.1 cells was observed within CD19⁺ B cells, CD11b⁺ myeloid cells, CD4⁺ T helper cells, and CD8⁺ T cytotoxic cells in the PB (F) and BM (G), within the Lin⁻, LSK, and SLAM HSC (H) and spleen (I).

(J and K) Myeloid and lymphoid lineage composition of CD45.2⁺ cells (left panel) and CD45.1⁺ cells (right panel) in the BM (J) and spleen (K) of CD45.2 mice.

The results are mean ± SEM, with n ≧ 10 biological replicates. In all the analyses, p less than 0.05 were considered significant (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. “ns” means non-significance).