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. 2022 Jun 23;185(13):2248–2264.e21. doi: 10.1016/j.cell.2022.04.039

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S3 — M-HSCT allows an efficient donor to recipient exchange of HSPCs within the human niche of hematochimeric mice, related to Figure 3

(A) Representative plots showing the gating strategy used to characterize the CXCR4^high population in the HSPC (CD34⁺CD133⁺CD90⁺) population.

(B) Scheme of CXCR4 cleavage following G-CSF mobilization and related antibodies localization.

(C) The percentage of migrating HSPCs, performed at 24 and 72 h post thawing, previously collected with G-CSF. A Kruskal-Wallis test was performed, followed by a post hoc analysis with Dunn’s test.

(D) The percentage of CXCR4^high cells (left panel) and MFI (right panel) over time after thawing, stained with an antibody targeting the ECL2 epitope of CXCR4, on the HSPC population mobilized with G-CSF or G-CSF/AMD3100.

(E) The percentage of CXCR4^high cells (left panel) and MFI (right panel) over time after thawing, stained with an antibody targeting the N terminus epitope of CXCR4, on the HSPC population mobilized with G-CSF.

(F) The percentage of KIT⁺ cells (left panel) and MFI (right panel) over time after thawing, on the HSPC population mobilized with G-CSF or G-CSF/AMD3100.

(G) The percentage of ITGA4⁺ cells (left panel) and MFI (right panel) over time after thawing, on the HSPC population mobilized with G-CSF or G-CSF/AMD3100. The longitudinal comparisons, performed by a mixed-effect model (REML), followed by a post hoc analysis with Dunnett’s test.

The results are mean ± SEM, with n ≧ 5, with 3 different donors. In all the analyses, p less than 0.05 were considered significant (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. “ns” means non-significance).