Table 1.
Estimated versus obtained chimerism following M-HSCT in Cd40lg−/− mice
| Treatment | Mobilized SLAM HSCs (mL) | Estimated mobilized SLAM HSCs/mouse (V blood = 1.5 mL) | Percentage of estimated BM vacancy | Total SLAM HSCs transplanted (from BM purified 2 × 106 Lin−) | Ratio recipient to donor | Percentage of estimated chimerism | Percentage of obtained chimerism |
|---|---|---|---|---|---|---|---|
| Sham | 10 | 15 | 0 | 4,000 | 1:0.00375 | 0 | N/A |
| G7 | 900 | 1,350 | 11 | 4,000 | 1:0.33 | 8 | N/A |
| G7A | 1,700 | 2,550 | 20 | 4,000 | 1:0.64 | 12 | 19 |
| G7AB | 3,500 | 5,250 | 42 | 4,000 | 1:1.31 | 18 | 30 |
| G7AB-H | 600 | 900 | 7 | 4,000 | 1:0.23 | 6 | 9 |
| G5(IP)A | 1,000 | 1,500 | 12 | 4,000 | 1:0.38 | 9 | N/A |
| G5(IP)AB | 2,500 | 3,750 | 30 | 4,000 | 1:0.94 | 15 | N/A |
| G3AB | 600 | 900 | 7 | 4,000 | 1:0.23 | 6 | 8 |
| G3AB-H | 350 | 525 | 4 | 4,000 | 1:0.13 | 4 | 5 |
| AB | 300 | 450 | 4 | 4,000 | 1:0.11 | 3 | N/A |
Estimation is based on the following theories: (1) mobilization occurred to the same extent throughout all the mouse BM, (2) the lower limbs account for 20% of it, and (3) the mobilized and infused cells compete equally (with n ≧ 5).