REAGENT	SOURCE	IDENTIFIER
Antibodies
Anti-human CD3	BD Biosciences	Cat# 555335; RRID:AB_398591
Anti-human CD3	BioLegend	Cat# 980004; RRID:AB_2632620
Anti-human CD3	BioLegend	Cat #300316; RRID:AB_314052
Anti-human CD4	BD Phosflow	Cat# 558116; RRID:AB_397037
Anti-human CD13	BD Biosciences	Cat# 557454; RRID:AB_398624
Anti-human CD13	Invitrogen	Cat# MHCD1304; RRID:AB_1730807
Anti-human CD19	BD Biosciences	Cat# 345789; RRID:AB_2868815
Anti-human CD19	BioLegend	Cat# 302216; RRID:AB_314246
Anti-human CD19	Miltenyi Biotec	Cat# 130-113-172; RRID:AB_2725999
Anti-human CD33	BD Biosciences	Cat# 333946; RRID:AB_399961
Anti-human CD33	Miltenyi Biotec	Cat# 130-099-485; RRID:AB_2660351
Anti-human CD34	BD phosflow	Cat# 348811; RRID:AB_2868855
Anti-human CD34	Miltenyi Biotec	Cat# 130-095-393; RRID:AB_10827793
Anti-human CD34	Miltenyi Biotec	Cat# 130-090-954; RRID:AB_244349
Anti-human CD38	BioLegend	Cat# 356614; RRID:AB_2562183
Anti-human CD38	Miltenyi Biotec	Cat# 130-113-428; RRID:AB_2733230
Anti-human CD45	BD Biosciences	Cat# 641417; RRID:AB_2800453
Anti-human CD45	BioLegend	Cat# 304029; RRID:AB_2174123
Anti-human CD45	BioLegend	Cat# 304016; RRID:AB_314404
Anti-human CD45	BioLegend	Cat# 304008; RRID:AB_314396
Anti-human CD45	Miltenyi Biotec	Cat# 130-091-230; RRID:AB_244233
Anti-human CD45RA	BioLegend	Cat# 304112; RRID:AB_314416
Anti-human CD45RA	BioLegend	Cat#304128; RRID:AB_10708880
Anti-human CD47	BD Biosciences	Cat# 563760; RRID:AB_2744414
Anti-human CD47	Miltenyi Biotec	Cat# 130-101-407; RRID:AB_2658401
Anti-human CD49d	BioLegend	Cat# 304304; RRID:AB_314430
Anti-human CD90	BD Biosciences	Cat# 555596; RRID:AB_2868855
Anti-human CD90	BD Biosciences	Cat# 559869; RRID:AB_398677
Anti-human CD117	BioLegend	Cat# 313212; RRID:AB_893222;
Anti-human CD184	Miltenyi Biotec	Cat# 130-109-844; RRID:AB_2655771
Anti-human CD184	Miltenyi Biotec	Cat# 130-117-519; RRID:AB_2734059
Anti-human CD184	Miltenyi Biotec	Cat# 130-109-845; RRID:AB_2655775
Anti-human CD184	ThermoFisher Scientific	Cat#TA591094
Anti-human CD184	ThermoFisher Scientific	Cat#TA591092
Anti-human CD133/2	Miltenyi Biotec	Cat#170-070-702; RRID:AB_10831361
Human Fc Block	Miltenyi Biotec	Cat# 130-059-901; RRID:AB_2892112
Anti-mouse CD3	BD Biosciences	Cat# 555275; RRID:AB_395699
Anti-mouse CD3	BD Biosciences	Cat# 555275; RRID:AB_395699
Anti-mouse CD3	BD Biosciences	Cat# 557596; RRID:AB_396759
Anti-mouse CD4	BD Biosciences	Cat# 558107; RRID:AB_397030
Anti-mouse CD4	BD Biosciences	Cat# 553051; RRID:AB_398528
Anti-mouse CD8	eBioscience	Cat# 47-0081-82; RRID:AB_1272185
Anti-mouse CD11b	BD Biosciences	Cat# 553312; RRID:AB_398535
Anti-mouse CD19	BioLegend	Cat# 115520; RRID:AB_313655
Anti-mouse CD19	BioLegend	Cat# 115526; RRID:AB_493341
Anti-mouse CD24	BD Biosciences	Cat# 562360; RRID:AB_11151895
Anti-mouse CD25	BD Biosciences	Cat# 551071; RRID:AB_394031
Anti-mouse CD44	BD Biosciences	Cat# 563970; RRID:AB_2738517
Anti-mouse CD45.1	BD Biosciences	Cat# 553776; RRID:AB_395044
Anti-mouse CD45.1	BD Pharmingen	Cat# 553775; RRID:AB_395043
Anti-mouse CD45.2	BD Pharmingen	Cat# 552950; RRID:AB_394528
Anti-mouse CD45.2	BioLegend	Cat# 109820; RRID:AB_492872
Anti-mouse CD45R/B220	BioLegend	Cat# 103224; RRID:AB_313007
Anti-mouse CD48	BioLegend	Cat# 103418; RRID:AB_756140
Anti-mouse CD48	BioLegend	Cat# 103406; RRID:AB_313021
Anti-mouse CD62L	BD Biosciences	Cat# 553152; RRID:AB_398533
Anti-mouse CD69	Fisher Scientific	Cat# 12-0691-81, RRID:AB_465731
Anti-mouse CD117	BD Pharmingen	Cat# 553356; RRID:AB_398536
Anti-mouse CD117	eBioscience	Cat# 47-1171-82; RRID:AB_1272177
Anti-mouse CD150	BioLegend	Cat# 115910; RRID:AB_493460
Anti-mouse CD184	ThermoFisher Scientific	Cat# 14-9991-82
Anti-mouse GL7	eBioscience	Cat# 50-5902-82; RRID:AB_2574252
Anti-mouse Lineage Cocktail	BioLegend	Cat# 133303; RRID:AB_1595553
Live/dead	Life Technologies	Cat# L-34959
Anti-mouse Ly6A/E (Sca1)	BD Biosciences	Cat# 558162; RRID:AB_647253
Anti-mouse Ly6A/E (Sca1)	BioLegend	Cat# 108120; RRID:AB_493273
Mouse Fc Block	BD Biosciences	Cat# 553142; RRID:AB_394657
Anti-mouse PNA	Vector Laboratories	Cat# FL-1071; RRID:AB_2315097
Anti-mouse TCRbeta	BioLegend	Cat# 109211; RRID:AB_313434
Chemicals, peptides, and recombinant proteins
5-Methoxyuridine-5'-Triphosphate	TriLink Biotechnologies	Cat# N-1093-10
5-Methylcytidine-5'-Triphosphate	TriLink Biotechnologies	Cat# N1014-10
ACK Lysing Buffer	ThermoFisher	Cat# A1049201
Alt-R Cas9 Electroporation Enhancer	Integrated DNA Technologies	Cat# 1075916
Alt-R CRISPR-Cas9 crRNA	Integrated DNA Technologies	Ferrari et al. 2021a
sNLS-SpCas9-sNLS Nuclease	Aldevron	Cat# 9212-5MG
AMD3100	Genzyme (Sanofi)	Mozobil
AMD3465	Sigma-Aldrich	Cat# SML1433-25MG
BIO5192	R&D System	Cat# 5051/10
CXCL12	PeproTech	Cat# 300-28A
CleanCap AG	Tebu-Bio	Cat#N-7113-5
G-CSF (Lenograstim)	Italfarmaco	Myelostim
hFlt3-L	PeproTech	Cat# 300-19
hSCF,	PeproTech	Cat# 300-07
hTPO	Tebu-bio	Cat# 300-18-0100
Imject Alum Adjuvant	ThermoFisher Scientifi	Cat# 77161
mFLT3-L	PeproTech	Cat# 250-31L
mSCF	PeproTech	Cat# 315-02
mTPO	PeproTech	Cat# 315-14
PGE2	Cayman Chemical Company	Cat# 39746-25-3
Pseudouridine-5'-Triphosphate	TriLink Biotechnologies	Cat# N-1019-10
SR1	BioVision	Cat# 1967-1
TNP-KLH (Keyhole Limpet Hemocyanin)	LGC Biosearch Technologies	Cat# T-5060
UM171	StemCell Technologies	Cat# 72914
Critical commercial assays
5X MEGAscript T7 kit	Life Technologies	Catt# AMB1334-5
Apoptosis Detection kit	BD Pharmingen	Cat# 559763
Lineage Cell Depletion Kit	Miltenyi Biotec	Cat# 130-090-858
Mouse Cell Depletion Kit	Miltenyi Biotec	Cat# 130-104-694
Mouse pro MMP9 ELISA Kit	Thermo Scientific	Cat# EMMP9
Mouse SDF-1 alpha / CXCL12 alpha ELISA Kit, for serum, plasma, and cell culture	Sigma-Aldrich	Cat# RAB0125-1KT
P3 Primary Cell 4D-Nucleofector X Kit	Lonza	Cat# V4XP-3032
TaqMan Gene Expression Assay	ThermoFisher Scientifi	Cat# 4331182
Experimental models: Cell lines
Human: G-CSF mPB CD34+ HSPCs	AllCells	N/A
Human: G-CSF/Mozobil mPB CD34+ HSPCs	AllCells	N/A
Human: HEK293T	ATCC	Cat# CRL-3216
Experimental models: Organisms/strains
C57Bl/6 Ly45.1	Charles River Laboratory.	Cat# 494
C57Bl/6 Ly45.2	Charles River Laboratory.	Cat# 027
Cd40lg-/- (B6.129S2-Cd40lgtm1Imx/J)	Jackson Laboratory	Cat# 002770; RRID:IMSR_JAX:002770
NSG	Charles River Laboratory	Cat# 614
NSGW41	Jackson Laboratory	Cat# 026497; RRID:IMSR_JAX:026497
Other
Micro-osmotic pump model 1003D	Alzet	Cat# 0000289
Micro-osmotic pump model 1007D	Alzet	Cat# 0000290
Transwell Permeable Support	Costar	Cat# 3421