TABLE 2.
P. carinii DNA in nonimmunosuppressed laboratory rats
| Rat sample no. | Presence of DNAa
|
|||||
|---|---|---|---|---|---|---|
| Single-round PCR
|
Nested PCR
|
|||||
| Any P. carinii (H + E)a | f. sp. carinii (RC1 + RC2) | f. sp. ratti (RR1 + RR2) | Any P. carinii (X + Z)b | f. sp. carinii (RC1 + RC2) | f. sp. ratti (RR1 + RR2) | |
| R1632 | + | + | − | + | + | − |
| R1633 | − | − | − | + | + | + |
| R1634 | − | − | − | − | − | − |
| R1635 | + | − | − | + | + | − |
| R1636 | − | − | − | + | + | − |
| R1637 | − | − | − | + | + | − |
| R1682 | − | NDb | ND | ND | − | − |
| R1683 | − | ND | ND | ND | + | − |
| R1684 | − | ND | ND | ND | + | − |
| R1685 | − | ND | ND | ND | + | − |
| R1686 | − | ND | ND | ND | + | − |
| R1687 | − | ND | ND | ND | + | − |
| No. positive/total | 2/12 | 1/6 | 0/6 | 5/6 | 10/12 | 1/12 |
a
H + E, primers pAZ102-H and pAZ102-E; +, present; −, absent; ND, not determined.
b
X + Z, primers pAZ102-X and pAZ102-Z.