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. 2022 Jan 31;7(3):222–236. doi: 10.1136/svn-2021-001308

Figure 4.

Figure 4

Disturbance of venous circulation decrease microvascular angiogenesis, and NBP treatment could improve angiogenesis. (A) Immunofluorescence staining of CD31 positive cells in the microvessels in the region of ischaemia from first to 14th day after operation in the sham, EJVO, MCAO, EJVO +MCAO and EJVO +MCAO+ NBP groups. (B, C) Western blots of expression of CD31. (D) Quantification of microvassels density measured by immunofluorescence staining of CD31. (E, F) Quantification analysis of relative expression of CD31 level measured by Western blot. Data are presented as mean±SD, n=5 per group for immunofluorescence staining and n=3 per group for Western bolt. *P＜0.05, MCAO vs sham; **P＜0.01, MCAO vs Sham; ## P＜0.01, EJVO +MCAO vs sham; §p＜0.05, EJVO +MCAO + NBP vs sham; §§p＜0.01, EJVO +MCAO + NBP vs sham; ※P＜0.05, MCAO vs EJVO; ※※P＜0.01, MCAO vs EJVO; & p＜0.05, EJVO +MCAO vs EJVO; &&p＜0.01, EJVO +MCAO vs EJVO; ∫∫p＜0.05, EJVO +MCAO vs MCAO; $p＜0.05, EJVO +MCAO + NBP vs EJVO +MCAO; $$p＜0.01, EJVO +MCAO + NBP vs EJVO +MCAO. Scale bar=50 µm. EJVO, external jugular vein occlusion; MACO, middle cerebral artery occlusion; NBP, Dl-3-n-butylphthalide.