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. 2022 Jun 29;81:104132. doi: 10.1016/j.ebiom.2022.104132

Figure 1.

Figure 1

Characterizing the cell types of undifferentiated hAOs and the permission of hAOs to 229E and OC43 infections. (A) to (D) Characterizing cell types by immunostaining Kertain 5 (basal cells), MUC5AC (goblet cells), β-tubulin (ciliated cells) and CC10 (club cells). (E) schematic diagram for seasonal coronaviruses inoculation in hAOs. (F) Dynamics of intracellular virus RNA copies in hAOs cultured at 33 °C from 1 to 24 h post-inoculation (n=3). (G) Dynamics of infectious viral titers in hAOs cultured at 33 °C from 1 to 24 h post-inoculation (n=3). (H) Immunofluorescence staining for viral double-stranded RNA (dsRNA) in hAOs at 33 °C. Scale bar = 25 μm. (I) Transmission electron microscopy analysis of 229E and OC43 infected hAOs cultured with expansion medium at 33 °C.