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. 2022 Jun 29;81:104132. doi: 10.1016/j.ebiom.2022.104132

Figure 2.

Figure 2

Recapitulating 229E and OC43 infections in differentiated hAOs. (A) Transcriptional levels of specific cell-type markers upon differentiation at 37 °C. (B) to (E) Characterizing cell types by immunostaining β-tubulin (ciliated cells), MUC5AC (goblet cells), Kertain 5 (basal cells) and CC10 (club cells) (Scale bar = 25 μm). (F) Dynamics of intracellular virus RNA copies in differentiated hAOs at 33 °C from 1 to 24 h post-inoculation (n=3). (G) Dynamics of infectious viral titers in differentiated hAOs at 33 °C from 1 to 24 h post-inoculation (n=3). (H) Immunofluorescence staining for viral double-stranded RNA (dsRNA) in differentiated organoids at 33 °C (Scale bar = 10 μm).