TABLE 1.
Relative abundance 1 of SDS-PAGE protein bands from the myofibrillar fraction of broiler breast fillets according to muscle condition (M—normal, NORM; Spaghetti Meat, SM) and postmortem time (T—0, 3, 7 days).
| Bands | Approximate molecular size and identification | Muscle condition (M) | Time (T) | SE | P-value | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| NORM | SM | 0 | 3 | 7 | M × T | M | T | |||
| 1 | Titin | 9.32 | 9.83 | 10.1 | 9.57 | 9.11 | 0.46 | NS | NS | NS |
| 2 | Nebulin | 1.85 | 1.83 | 2.70 a | 1.73 b | 1.08 b | 0.34 | NS | NS | 0.0001 |
| 3 | 220 kDa, MyHC | 39.8 | 40.6 | 38.7 | 40.4 | 41.5 | 2.0 | NS | NS | NS |
| 4 | 150 kDa, MyBPC | 2.46 | 2.30 | 2.95 | 2.24 | 1.94 | 0.48 | NS | NS | NS |
| 5 | 105 kDa, α-actinin | 2.16 | 2.03 | 2.42 a | 2.14 ab | 1.74 b | 0.25 | NS | NS | 0.0053 |
| 6 | 80 kDa | 1.00 a | 0.81 b | 0.91 | 0.94 | 0.87 | 0.15 | NS | 0.0057 | NS |
| 7 | 53 kDa, desmin | 0.85 | 0.85 | 0.84 | 0.82 | 0.89 | 0.08 | NS | NS | NS |
| 8 | 42 kDa, actin | 21.8 | 21.3 | 21.4 | 21.4 | 21.7 | 1.0 | NS | NS | NS |
| 9 | 38 kDa, TnT | 5.04 | 5.01 | 5.08 | 5.04 | 4.95 | 0.49 | NS | NS | NS |
| 10 | 28–30 kDa | 0.18 | 0.16 | 0.12 b | 0.27 a | 0.12 b | 0.05 | NS | NS | <0.0001 |
| 11 | 21 kDa, MLC-1 | 1.55 | 1.34 | 0.82 b | 1.33 b | 2.18 a | 0.42 | NS | NS | <0.0001 |
| 12 | 17 kDa, MLC-2 | 3.68 | 3.42 | 4.14 a | 3.46 b | 3.05 b | 0.43 | NS | NS | 0.0005 |
| 13 | 15 kDa, MLC-3 | 1.51 | 1.75 | 1.45 | 1.68 | 1.76 | 0.30 | NS | NS | NS |
1
Data expressed as individual protein band abundance as a percentage of total protein abundance in the entire lane. (MyHC, myosin heavy chain; MyBPC, myosin binding protein C; TnT, troponin T; MLC-1, myosin light chain 1; TnI, troponin I; TnC, troponin C; MLC-2, myosin light chain two; MLC-3, myosin light chain 3).
ab
Means within the same row and effect followed by different superscripts differ p < 0.05.