TABLE 3.
Name | Reverse | Forward |
18s | 5′-CCTCAGTTCCGAAAACCAAC-3′ | 5′-ACCGCAGCTAGGAATAATGG-3′ |
Fasn | 5′-GGTCGTTTCTCCATTAAATTCTCAT-3′ | 5′-CTAGAAACTTTCCCAGAAATCTTCC-3′ |
SREBP-1c | 5′-TAGATGGTGGCTGCTGAGTG-3′ | 5′-GATCAAAGAGGAGCCAGTGC-3′ |
TNFα | 5′-CCACAAGCAGGAATGAGAAGA-3′ | 5′-ACGTGGAACTGGCAGAAGAG-3′ |
HSL | 5′-TGCCCAGGAGTGTGTCTGAG-3′ | 5′-AGGACACCTTGGCTTGAGCG-3′ |
ATGL | 5′-GGTTCAGTAGGCCATTCCTC-3′ | 5′-GTGCAAACAGGGCTACAGAG-3′ |
CPT-1 | 5′-CAGCGAGTAGCGCATAGTCA-3′ | 5′-TGAGTGGCGTCCTCTTTGG-3′ |
IL-6 | 5′-TGCAAGTGCATCATCGTTGT-3′ | 5′-ACTTCACAAGTCGGAGGCTTAAT-3′ |
PPARγ | 5′-CAGCTTCTCCTTCTCGGCCT-3′ | 5′-CACAATGCCATCAGGTTTGG-3′ |
PGC-1α | 5′-AGAGCAAGAAGGCGACACAT-3′ | 5′-AACAAGCACTTCGGTCATCC-3′ |
PPAR α | 5′-CTGCGCATGCTCCGTG-3′ | 5′-CTTCCCAAAGCTCCTTCAAAAA-3′ |
18S, 18S ribosomal RNA; Fasn, fatty acid synthase gene; SREBP-1c, sterol regulatory element-binding transcription factor 1; TNFα, tumor necrosis factor alpha; HSL, hormone sensitive lipase; ATGl, adipose triglyceride lipase; CPT-1, carnitine palmitoyl transferase I; IL-6, interleukin 6; PPARγ, peroxisome proliferator-activated receptor gamma; PGC-1 α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PPAR α, peroxisome proliferator-activated receptor alpha.