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(A) Superimposed HPLC-DAD chromatograms at 282 nm of ergosterol extracted from C. albicans treated with miconazole (1.5 μg/mL) and SAN (6.25, 12.5, and 25.0 μg/mL). (B) Relative HPLC peak areas of ergosterol [Retention time (RT) = 28.7 min]. (C) The comparison of UV Spectra data between the peak 1 (standard ergosterol) and 1' at RT = 28.7 min. The data are presented as the mean ± SEM (n = 4, ***p < 0.001 compared to negative control, ANOVA).
