Table 1.
Fluorescence quenching constants, bimolecular quenching rate constant, apparent binding constants, binding site numbers and correlation coefficients of SPI-LUT nanodelivery system at different temperatures.
| Sample | Temperature/K | Ksv (×104 L⋅mol−1⋅s−1) | Kq (×1012 L⋅mol−1⋅s−1) | R12 | Ks/M | n | R22 |
|---|---|---|---|---|---|---|---|
| SPI-LUT-0 | 298 | 7.1045 | 7.1045 | 0.9851 | 109.4460 | 1.6535 | 0.9714 |
| 306 | 4.3422 | 4.3422 | 0.9860 | 26.7454 | 1.4701 | 0.9707 | |
| 314 | 3.4744 | 3.4744 | 0.9761 | 21.4981 | 1.3919 | 0.9960 | |
| SPI-LUT-100 | 298 | 3.0691 | 3.0691 | 0.9791 | 5943.1953 | 2.0098 | 0.9919 |
| 306 | 3.0159 | 3.0159 | 0.9955 | 502.2501 | 1.7820 | 0.9961 | |
| 314 | 2.6792 | 2.6792 | 0.9957 | 840.1201 | 1.6129 | 0.9849 | |
| SPI-LUT-200 | 298 | 4.9595 | 4.9595 | 0.9472 | 1501.2741 | 1.8853 | 0.9962 |
| 306 | 4.8809 | 4.8809 | 0.9403 | 1989.7016 | 2.0169 | 0.9957 | |
| 314 | 4.7629 | 4.7629 | 0.9437 | 1411.6924 | 1.9632 | 0.9965 | |
| SPI-LUT-300 | 298 | 5.0767 | 5.0767 | 0.9397 | 3002.6865 | 1.8874 | 0.9693 |
| 306 | 4.5576 | 4.5576 | 0.9649 | 4288.9442 | 2.0080 | 0.9910 | |
| 314 | 3.9747 | 3.9747 | 0.9756 | 515.8816 | 1.7924 | 0.9881 | |
| SPI-LUT-400 | 298 | 1.7279 | 1.7279 | 0.9699 | 470.8954 | 1.1126 | 0.9820 |
| 306 | 1.5698 | 1.5698 | 0.9977 | 863.6455 | 1.1914 | 0.9983 | |
| 314 | 1.5804 | 1.5804 | 0.9805 | 1910.3669 | 1.2791 | 0.9913 | |
| SPI-LUT-500 | 298 | 1.9711 | 1.9711 | 0.9479 | 2117.7329 | 1.2664 | 0.9619 |
| 306 | 1.1178 | 1.1178 | 0.9277 | 890.3204 | 0.6906 | 0.9688 | |
| 314 | 1.0019 | 1.0019 | 0.9162 | 430.2395 | 0.6240 | 0.9700 |