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. 2022 Jun 29;12(29):18848–18863. doi: 10.1039/d1ra09396g

Benefits and limitations of biomass21,22,25.

Feedstock Benefits Limitations
First-generation feedstock
Starch and sucrose feedstocks High butanol yield Occupies potential crop land
Sufficient fermentable substrates through simple pretreatment processes Competes with food supply
Significantly increases the overall cost of fermentation
Second-generation feedstock
Lignocellulose biomass Cost-effective, huge carbon resources Difficult to achieve sufficient fermentable sugars from complex and recalcitrant biomass
No competition with food supply Requires complex pretreatment and detoxification processes
Third-generation feedstock
Algal biomass No competition with cultivation land and food supply Significantly increases the cost of downstream processes due to low production and productivity of butanol
Fewer or no lignin and fermentation inhibitors Difficult to obtain sufficient fermentable sugars
Fourth-generation feedstock
Syngas Increased CO2 capture ability Still at its infancy as a technology
No complex pretreatment process needed Several unknown key parameters that limit butanol production
Directly utilises clostridia with a high production rate Poor mass transfer from gas to liquid