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. 2022 Jun 13;10:860138. doi: 10.3389/fbioe.2022.860138

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Copyright © 2022 Schumacher, Roumans, Rademakers, Joris, Eischen-Loges, van Griensven and LaPointe.

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Kidney organoids cultured in both 7 and 21% O₂ show differential nuclear translocation of HIF1a and HIF2a. (A). Immunofluorescence staining for HIF1a (red) and DAPI (blue) shows increased nuclear translocation in podocytes (composite), particularly in 7% O₂ at day 7 + 25. Scale bar: 50 µm. (B). HIF2a (red) and DAPI (blue) immunofluorescence staining shows nuclear translocation in most cells within the organoid irrespective of the oxygen concentration or time point. (n = 3, N = 3). Scale bar: 50 µm. Arrowheads indicate the (air-exposed) surface of the organoids.