FIG. 4.

Release of saccharide and protein during enzymatic degradation of sheath. Sheath medium supplemented with crude enzyme was incubated at 30°C. At intervals of 24 h, the amounts of soluble saccharide (●, based on glucose), protein (■, based on bovine serum albumin), and residual sheath (▴) were measured. Heat-denatured (95°C, 30 min) enzyme was prepared and incubated with sheath in the same manner as the control, with the amounts of saccharide (○), protein (□), and sheath (▵) being measured.