TABLE 3.
Inhibitorb | Relative activity (%) |
---|---|
None | 100 |
MIA | 73 |
IAA | 240 |
DTT | 0 |
PMSF | 107 |
NaN3 | 108 |
DFP | 97 |
EDTA | 108 |
Sheath polysaccharide-degrading activity was assayed under standard conditions, with 1 mM as the final concentration of inhibitors. The activity without the addition of any inhibitors was defined as 100%.
Abbreviations: MIA, monoiodoacetic acid; IAA, iodoacetamide; DTT, dithiothreitol; PMSF, phenylmethylsulfonyl fluoride; NaN3, sodium azide; DFP, diisopropylfluorophosphate.