Fig. 1.

Cisplatin promotes renal ferroptosis and downregulates FXR expression in mice.

The serum and the kidneys were collected from mice after 48 h and 72 h of cisplatin injection (20 mg/kg), (n = 6–7). (A) Serum BUN and Cr levels of each group. (B) Protein levels of GPX4, HMOX1, and FXR were detected by immunoblotting. The relative protein levels are shown. The values for the control group were set to 1. (C) Representative images of hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining to examine the histology and immunohistochemistry to examine the expression of 3-NT and 4-HNE. Scale bar, 100 μm. Computer-based morphometric analysis is shown (right bar graph, n = 7–8 in each group). All values are presented as the mean ± SD. Statistical significance was measured using one-way ANOVA with Bonferroni post hoc-test. *P < 0.05, **P < 0.005.