Fig. 8.

FXR epigenetically activates ferroptosis-related genes.

WT and FXR KO mice were fasted overnight (16 h) and were subsequently administered GW4064 or vehicle by oral gavage. After 1 h or 4 h of GW4064 administration, ChIP assay was performed using mice kidney tissues. (A and C) Experimental outline. (B) ChIP assay was performed after treatment with GW4064 for 1 h. Occupancy of FXR at the indicated genes (n = 4 mice). (D–E) ChIP assay was performed after treatment with GW4064 for 4 h. Occupancy of FXR (D) and MAFG (E) at the indicated genes (n = 4 mice). All values are presented as the mean ± SD. Statistical significance was measured using one- or two-way ANOVA with Bonferroni post hoc-test. *P < 0.05, **P < 0.005.