Figure 4.

Extracting corrected B2 unfolding parameters from biased AFM-SMFS data. (a) AFM experimental setup. (b, c) Representative force traces and the aligned contour length histograms showing the two possible dissociation pathways for the SdrG-B2 system. (d) Dynamic force spectrum of the biased B2 unfolding force (orange) and Sdrg:FgB rupture events (blue). The most probable rupture forces and loading rates were fitted using the Bell–Evans model shown in dashed lines. We used the fitting approach based on minimizing residuals of η to obtain a corrected Bell–Evans expression for the loading rate dependency of B2 domain unfolding (black dashed line) and the corresponding unfolding force distributions (right, solid black lines).