Figure 5.

Monte Carlo simulations showing mechanical work (energy) dissipation as a function of the relative mechanical stability of the FP domain and the loading rate. Monte Carlo simulations under a constant loading rate were conducted on both (a) C. perfringens cohesin-dockerin RL system with variable stability of the FIVAR FP and (b) S. epidermidis SdrG:FgB RL system with variable stability of the B2 domain FP. The FP has a fixed initial off-rate k_o and the unfolding force distribution was tuned by adjusting both the energy barrier position (Δx) and the loading rate from 1 to 10¹⁰ pN/s; 5000 simulations were performed for each energy barrier position and loading rate. The simulations corresponding to the WT sacrificial (a) FIVAR and (b) B2 are shown on the plots as black dots. Two-dimensional (2D) cutouts from these three-dimensional (3D) surface plots are shown in Supporting Information Figure 5.