FIGURE 1.

Voluntary chronic running to midlife is not different between apolipoprotein E (APOE) genotypes. A, Schematic of the voluntary running paradigm in which APOE ε3/ε3, APOE ε3/ε4, and APOE ε4/ε4 male and female mice were introduced to a locked (control – sedentary) or unlocked (treatment – running) running wheel at 1 month until 12 months (midlife). Longitudinal, advanced, and post mortem phenotyping is indicated. B‐C, No difference in running (average rotations across multiple consecutive nights) between APOE genotypes at both 6 and 11 months in female (B) or male (C) mice (n = 10–15). D‐G, Average rotations per mouse at 6 and 12 months showed an age‐dependent decrease for both females (D) and males (F); however, the change over time was not significantly different between genotypes (E,G) (n = 10–15). Data presented as mean ± standard deviation, one‐way analysis of variance with Tukey's multiple comparison performed for B,C,E,G. Two‐sided paired t‐test performed for D,F. *P < .05, **P < .01